Differential association and distribution of acetyl- and butyrylcholinesterases within rat liver subcellular organelles.
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Subcellular distribution and properties of kynurenine transaminase in rat liver.
Kynurenine transaminase activity in rat liver was found in both the mitochondrial and supernatant fractions. The mitochondrial and supernatant fractions contained (a) kynurenine-pyruvate transaminase, which showed a preference for pyruvate as amino acceptor and a pH optimum between 8.0 and 8.5, and (b) kynurenine-alpha-oxoglutarate transaminase, with a preference for alpha-oxoglutarate and a pH...
متن کاملDifferential transglutaminase distribution in normal rat liver and rat hepatoma.
Distribution of transglutaminase activity was determined in normal rat liver, a 3'-methyl-4-dimethylaminoazobenzene-induced primary hepatoma, and the Novikoff hepatoma. Over 90% of the total enzyme activity was found in the 105,000 X g supernatant of normal liver, whereas only 30% was found in this fraction of the hepatomas, the remainder being found in the particulate fraction. The is distribu...
متن کاملDistribution of metals in subcellular fractions of rat liver.
The localization of various metabolic processes of cells in particulate and soluble intracellular components has been studied intensively (l-3). The effect of added metals on these processes has also received attention (4-l3), but information on the metal content of the cell fractions is limited (14-16). The association of metals with proteins has been divided into two categories on the basis o...
متن کاملthe differential effects of debate and media analysis as relate to enhancing efl learners critical thinking ability and writing performances
critical thinking ability has an important role in education. accordingly, scholars around the world are searching for new ways for teaching and improving students critical thinking ability. in line with the studies in efl contexts supporting the positive relationship between critical thinking and writing performances, this study investigated the differential effects of halverson’s critical thi...
15 صفحه اولSubcellular distribution of superoxide dismutases in rat liver.
Rat liver was homogenized in isotonic buffer, fractionated by differential centrifugation, and then subfractionated by equilibrium sedimentation in Nycodenz gradients. Fractions were assayed for both Cu,Zn-SOD and Mn-SOD by exploiting the cyanide-sensitivity of the former activity and by the use of specific antibodies. As expected, the cytosol and lysosomal fractions contained Cu,Zn-SOD; while ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)40218-4